SYNTHESIS OF CELLULOSE FROM PYRUVATE BY SUCCINATE-GROWN CELLS OF ACETOBACTER XYLINUM
نویسندگان
چکیده
منابع مشابه
Synthesis of Cellulose by Acetobacter Xylinum
Appearance of cellulose microfibrils in the medium of a suspension of cells of Acetobacter xylinum in buffered glucose solution was preceded by a stage during which the cellulose in the medium was amorphous within the available resolution. The size of the vertical axis of the microfibrils of the bacterial cellulose was found on the basis of measurement of shadow length to be only about 16 A. In...
متن کاملSynthesis of cellulose by Acetobacter Xylinum. 4. Enzyme systems present in a crude extract of glucose-grown cells.
Brown, S. A. & Neish, A. C. (1954). Canad. J. Biochem. Physiol. 32, 170. Butlin, K. R. (1938). Biochem. J. 32, 508.. Edelman, J., Ginzburg, V. & Hassid, W. Z. (1955). J. biol. Chem. 213, 843. Fitting, C. & Doudoroff, M. (1952). J. biol.'Chem. 199, 153. Friedemann, T. E. & Haugen, G. E. (1943). J. biol. Chem. 147, 415. Greathouse, G. A. (1953). Science, 117, 553. Greathouse, G. A., Shirk, H. G. ...
متن کاملAlternative Environmental Roles for Cellulose Produced by Acetobacter xylinum.
The cellulose-producing bacterium Acetobacter xylinum has been considered a strict aerobe, and it has been suggested that the function of cellulose is to hold cells in an aerobic environment. In this study, we showed that A. xylinum is capable of growing microaerophilically. Cellulose pellicles provided significant protection to A. xylinum cells from the killing effects of UV light. In experime...
متن کاملIn vitro synthesis of cellulose II from a cytoplasmic membrane fraction of Acetobacter xylinum.
The cytoplasmic and outer membranes of Acetobacter xylinum (ATCC 53582) were isolated by discontinuous sucrose density ultracentrifugation. Both lysozyme (EC 3.2.1.17) and trypsin (EC 3.4.21.4) were required for efficient crude membrane separation. Primary dehydrogenases and NADH oxidase were used as cytoplasmic membrane markers, and 2-keto-3-deoxyoctulosonic acid was used to identify the outer...
متن کاملFactors afecting the activity of pyruvate kinase of Acetobacter xylinum.
1. Extracts of Acetobacter xylinum were found to contain the glycolytic enzymes involved in the conversion of triose phosphate into pyruvate. Pyruvate kinase had the lowest relative activity. Phosphofructokinase activity was not detected in the extracts. 2. Only slight differences in the activity of pyruvate kinase were observed between cells grown on glucose and those grown on intermediates of...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1962
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.84.4.625-630.1962